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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two more splice variants had been claimed but specific nucleotide sequence info is not really nonetheless offered.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This tutorial outlines even more feasible optimization of mobile and immunoassay parameters to make certain the best possible effects are...
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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to ensure the best possible final results are...
Antibodies certain to the blocking peptide no more bind to your epitope within the focus on protein. By comparing the staining from the blocked antibody compared to the antibody alone, you can see which staining is unique.
Target Information Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation styles which lead towards the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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